Reverse Phase

HYDROCELL RP 5S and RP 10S are produced from PS-DVB particles, which have high pore volume and large pore size. The major advantages of these media are lower back pressure and higher permeability in the operation. The media also reduce the back pressure fluctuation when they are used in the gradient separations.

- RP 5S is prepared from 5 µm (d50), average 500 Å spherical PS-DVB particles. The media provide optimum pore volume and pore size with increasing permeability and high separating efficiency for small peptides, nucleotides and other small biomolecules.

- RP 10S is prepared from 10 µm (d50), average 500 Å spherical PS-DVB particles, which give increased permeability and high separating efficiency. It is suitable for the separation of polypeptides, oligonucleotide and other biomolecules.

Click for more information and Chromatograms 

HYDROCELL RP 5G and RP 10G are produced from highly cross-linked polystyrene-divinyl benzene (PS-DVB) spherical beads. The surface of the materials has been modified by hydrophilic coating to reduce the hydrophobicity. The small pores of these particles have been sealed completely to prevent the trapping of biomolecules.

- RP 5G is prepared from 5 µm, 500 Å spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiency for small proteins, polypeptides and oligonucleotides.

- RP 10G is prepared from 10 µm, 500 Å spherical PS-DVB particles with hydrophilic surface coating. This polymer provides optimum surface area with high separating efficiency for polypeptides, proteins and oligonucleotides.

These materials are extremely stable and permit use of mobile phase pH from 1 to 14 and the maximum temperature limit is up to 80°C. Reversed phase chromatography is based on the discrimination by hydrophobicity of biomolecules and is a complementary technique to ion exchange and hydrophobic interaction chromatography.